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Image Search Results
Journal: Molecular Biology of the Cell
Article Title: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function
doi: 10.1091/mbc.E20-07-0464
Figure Lengend Snippet: TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit anti–claudin-3 pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Article Snippet: Rabbit anti–ZO-1 pAb (#61-7300), rabbit anti-tricellulin mAb (clone 54H19L38; #700191), rabbit anti–claudin-2 pAb (#51-6100), rabbit
Techniques: Fluorescence, Microscopy, Staining
Journal: Molecular Biology of the Cell
Article Title: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function
doi: 10.1091/mbc.E20-07-0464
Figure Lengend Snippet: Establishment of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells. (A, B) Ctrl, Tric -KO #1, and Tric / Ocln -dKO #1 cells (A) and Ctrl, Ocln -KO #1, and Tric / Ocln -dKO #1 cells (B) were immunostained with rat anti-tricellulin mAb (A) or rat anti-occludin mAb (B) (green) and rabbit anti-ZO-1 pAb (red). All cells were stained with DAPI (blue). Note that tricellulin signal (white arrowheads) is absent in the Tric -KO and Tric / Ocln -dKO cells (magenta arrowheads) in A. Scale bars, 20 µm. (C) Immunoblotting of the total cell lysates of Ctrl, Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells using rabbit anti-tricellulin mAb and rabbit anti-occludin pAb. Asterisks on the occludin panel indicate nonspecific bands. β-Actin served as a loading control.
Article Snippet: Rabbit
Techniques: Staining, Western Blot
Journal: Molecular Biology of the Cell
Article Title: Occludin and tricellulin facilitate formation of anastomosing tight-junction strand network to improve barrier function
doi: 10.1091/mbc.E20-07-0464
Figure Lengend Snippet: TJs of Tric -KO, Ocln -KO, and Tric / Ocln -dKO cells appear normal in fluorescence microscopy. (A–D) Ctrl, Tric -KO #1, Ocln -KO #1, or Tric / Ocln -dKO #1 cells were mixed and cocultured with Ctrl-GFP-nls cells (green). Cells were immunostained with rat anti-ZO-1 mAb (A), mouse anti-cingulin mAb (B), mouse anti–claudin-1 mAb (C) or rabbit anti–claudin-3 pAb (D) (red) and stained with DAPI (blue). Scale bars, 20 µm. (E) Quantification of fluorescence intensity of claudin-3 at cell–cell junctions. The intensity at each cell–cell junction in the Ctrl (dark blue closed circles), Tric -KO #1 (orange closed circles), Ocln -KO #1 (green closed circles), and Tric / Ocln -dKO #1 cells (magenta closed circles) were normalized to the averaged intensity in the Ctrl-GFP-nls cells (lime-green open circles) and plotted against the length of the cell–cell junctions. n = 158 and 120 (Ctrl), 202 and 134 ( Tric -KO), 160 and 139 ( Ocln -KO), and 209 and 136 ( Tric / Ocln -dKO). *** p < 0.001 in Welch’s t test of junction-length-weighted average of the fluorescence intensity.
Article Snippet: Rabbit
Techniques: Fluorescence, Microscopy, Staining